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hedgehog pathway inhibitor cyclopamine  (MedChemExpress)


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    MedChemExpress hedgehog pathway inhibitor cyclopamine
    RPL35 regulates the chondrocyte phenotype in osteoarthritis via the hedgehog (Hh) pathway. (A) Venn diagram analysis of mouse primary chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA (Si-RPL35) for 48 h. (B) The bubble plots showing KEGG pathway enrichment data for genes that were up-regulated. (C) Quantitative PCR analysis of RPL35, Gli-1, SMO in chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA for 48 h n = 6 per group. (D, E) Immunostaining and quantification of Gli-1, PTCH1 in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (F) Immunofluorescent staining and quantification of SMO in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (G) Western blotting analysis of Gli-1, SMO, PTCH1 in normal primary chondrocytes, RPL35 siRNA-transfected chondrocytes, and mechanical loading chondrocytes treated with or without Ad-RPL35 for 48 h; The GAPDH was used as a loading control. n = 3 per group. (H) Western blotting analysis of Gli-1, MMP13, P16, and P21 in mouse primary chondrocytes treated with RPL35 siRNA for 48 h with or without <t>cyclopamine</t> (10 μM) for 24 h n = 3 per group. *P < 0. 05, **P < 0. 01, ***P < 0. 001 , ns not significant.
    Hedgehog Pathway Inhibitor Cyclopamine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 72 article reviews
    hedgehog pathway inhibitor cyclopamine - by Bioz Stars, 2026-02
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    1) Product Images from "RPL35 downregulated by mechanical overloading promotes chondrocyte senescence and osteoarthritis development via Hedgehog-Gli1 signaling"

    Article Title: RPL35 downregulated by mechanical overloading promotes chondrocyte senescence and osteoarthritis development via Hedgehog-Gli1 signaling

    Journal: Journal of Orthopaedic Translation

    doi: 10.1016/j.jot.2024.01.003

    RPL35 regulates the chondrocyte phenotype in osteoarthritis via the hedgehog (Hh) pathway. (A) Venn diagram analysis of mouse primary chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA (Si-RPL35) for 48 h. (B) The bubble plots showing KEGG pathway enrichment data for genes that were up-regulated. (C) Quantitative PCR analysis of RPL35, Gli-1, SMO in chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA for 48 h n = 6 per group. (D, E) Immunostaining and quantification of Gli-1, PTCH1 in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (F) Immunofluorescent staining and quantification of SMO in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (G) Western blotting analysis of Gli-1, SMO, PTCH1 in normal primary chondrocytes, RPL35 siRNA-transfected chondrocytes, and mechanical loading chondrocytes treated with or without Ad-RPL35 for 48 h; The GAPDH was used as a loading control. n = 3 per group. (H) Western blotting analysis of Gli-1, MMP13, P16, and P21 in mouse primary chondrocytes treated with RPL35 siRNA for 48 h with or without cyclopamine (10 μM) for 24 h n = 3 per group. *P < 0. 05, **P < 0. 01, ***P < 0. 001 , ns not significant.
    Figure Legend Snippet: RPL35 regulates the chondrocyte phenotype in osteoarthritis via the hedgehog (Hh) pathway. (A) Venn diagram analysis of mouse primary chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA (Si-RPL35) for 48 h. (B) The bubble plots showing KEGG pathway enrichment data for genes that were up-regulated. (C) Quantitative PCR analysis of RPL35, Gli-1, SMO in chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA for 48 h n = 6 per group. (D, E) Immunostaining and quantification of Gli-1, PTCH1 in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (F) Immunofluorescent staining and quantification of SMO in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (G) Western blotting analysis of Gli-1, SMO, PTCH1 in normal primary chondrocytes, RPL35 siRNA-transfected chondrocytes, and mechanical loading chondrocytes treated with or without Ad-RPL35 for 48 h; The GAPDH was used as a loading control. n = 3 per group. (H) Western blotting analysis of Gli-1, MMP13, P16, and P21 in mouse primary chondrocytes treated with RPL35 siRNA for 48 h with or without cyclopamine (10 μM) for 24 h n = 3 per group. *P < 0. 05, **P < 0. 01, ***P < 0. 001 , ns not significant.

    Techniques Used: Real-time Polymerase Chain Reaction, Immunostaining, Injection, Staining, Western Blot, Transfection, Control



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    RPL35 regulates the chondrocyte phenotype in osteoarthritis via the hedgehog (Hh) pathway. (A) Venn diagram analysis of mouse primary chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA (Si-RPL35) for 48 h. (B) The bubble plots showing KEGG pathway enrichment data for genes that were up-regulated. (C) Quantitative PCR analysis of RPL35, Gli-1, SMO in chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA for 48 h n = 6 per group. (D, E) Immunostaining and quantification of Gli-1, PTCH1 in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (F) Immunofluorescent staining and quantification of SMO in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (G) Western blotting analysis of Gli-1, SMO, PTCH1 in normal primary chondrocytes, RPL35 siRNA-transfected chondrocytes, and mechanical loading chondrocytes treated with or without Ad-RPL35 for 48 h; The GAPDH was used as a loading control. n = 3 per group. (H) Western blotting analysis of Gli-1, MMP13, P16, and P21 in mouse primary chondrocytes treated with RPL35 siRNA for 48 h with or without <t>cyclopamine</t> (10 μM) for 24 h n = 3 per group. *P < 0. 05, **P < 0. 01, ***P < 0. 001 , ns not significant.
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    RPL35 regulates the chondrocyte phenotype in osteoarthritis via the hedgehog (Hh) pathway. (A) Venn diagram analysis of mouse primary chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA (Si-RPL35) for 48 h. (B) The bubble plots showing KEGG pathway enrichment data for genes that were up-regulated. (C) Quantitative PCR analysis of RPL35, Gli-1, SMO in chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA for 48 h n = 6 per group. (D, E) Immunostaining and quantification of Gli-1, PTCH1 in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (F) Immunofluorescent staining and quantification of SMO in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (G) Western blotting analysis of Gli-1, SMO, PTCH1 in normal primary chondrocytes, RPL35 siRNA-transfected chondrocytes, and mechanical loading chondrocytes treated with or without Ad-RPL35 for 48 h; The GAPDH was used as a loading control. n = 3 per group. (H) Western blotting analysis of Gli-1, MMP13, P16, and P21 in mouse primary chondrocytes treated with RPL35 siRNA for 48 h with or without cyclopamine (10 μM) for 24 h n = 3 per group. *P < 0. 05, **P < 0. 01, ***P < 0. 001 , ns not significant.

    Journal: Journal of Orthopaedic Translation

    Article Title: RPL35 downregulated by mechanical overloading promotes chondrocyte senescence and osteoarthritis development via Hedgehog-Gli1 signaling

    doi: 10.1016/j.jot.2024.01.003

    Figure Lengend Snippet: RPL35 regulates the chondrocyte phenotype in osteoarthritis via the hedgehog (Hh) pathway. (A) Venn diagram analysis of mouse primary chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA (Si-RPL35) for 48 h. (B) The bubble plots showing KEGG pathway enrichment data for genes that were up-regulated. (C) Quantitative PCR analysis of RPL35, Gli-1, SMO in chondrocytes treated with 20 % elongation strain loading for 24 h or RPL35 siRNA for 48 h n = 6 per group. (D, E) Immunostaining and quantification of Gli-1, PTCH1 in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (F) Immunofluorescent staining and quantification of SMO in articular cartilage of mice intra-articularly injected with or without Ad-RPL35 for 4 weeks under mechanical loading. n = 5 per group, Scale bar: 50 μm. (G) Western blotting analysis of Gli-1, SMO, PTCH1 in normal primary chondrocytes, RPL35 siRNA-transfected chondrocytes, and mechanical loading chondrocytes treated with or without Ad-RPL35 for 48 h; The GAPDH was used as a loading control. n = 3 per group. (H) Western blotting analysis of Gli-1, MMP13, P16, and P21 in mouse primary chondrocytes treated with RPL35 siRNA for 48 h with or without cyclopamine (10 μM) for 24 h n = 3 per group. *P < 0. 05, **P < 0. 01, ***P < 0. 001 , ns not significant.

    Article Snippet: Primary chondrocytes were treated with RPL35 siRNA and 10 μM hedgehog pathway inhibitor cyclopamine (MCE, Monmouth Junction, NJ, USA).

    Techniques: Real-time Polymerase Chain Reaction, Immunostaining, Injection, Staining, Western Blot, Transfection, Control